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Plant Regeneration from Seed Culture of Cucumis Dipsaceus Ehrenb
Siva Subramanian S1*., Krishnapriya A1. And Sivalingam R2

Published in: Journal for Advance Research in Applied Sciences
Volume- 5, Issue-1, pp.10-13, Jan 2018
DPI :-> 16.10089.JARAS.2018.V5I1.1013.2272



Abstract
The present paper focuses on in vitro regeneration method for the tendril climber and economic important medicinal plant Cucumis dipsaceus Ehrenb. Was established. The seeds were treated with GA3 produced in vitro grown seedlings within ten days intervals. Among the different treatments water as well as GA3 induced the highest percentage 86% maturation and germination of embryos. The in vitro generated shoots turned brown at base and gradually turned black resulting in reduced plantlets rooting. Addition pinch of charcoal to the rooting medium resulted in increased rooting percentage. The rooted plants, transferred to a soil mix had a survival rate of 91% in the green house. All the plants that were transferred to wetlands survived.

Key-Words / Index Term
In vitro, medicinal plant, hardening

How to cite this article
Siva Subramanian S1*., Krishnapriya A1. And Sivalingam R2 , “Plant Regeneration from Seed Culture of Cucumis Dipsaceus Ehrenb”, Journal for Advance Research in Applied Sciences, 5, Issue-1, pp.10-13, Jan 2018. DPI:16.10089.JARAS.V5.I1.2272