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IN VITRO PROPAGATION OF ANISOCHILUS CARNOSUS (Lf.) Wallich – A MEDICINAL PLANT
Siva Subramanian S., Krishnapriya A. And Narayanayyar V

Published in: Journal for Advance Research in Applied Sciences
Volume- 4, Issue-7, pp.245-247, Dec 2017
DPI :-> 16.10089.JARAS.2017.V4I7.245247.2248



Abstract
An efficient protocol is described for the rapid in vitro multiplication of an important medicinal plant, Anisochilus carnosus (Lf.) Wallich (Lamiaceae) enhanced axillary bud proliferation from nodal explants collected from young shoots of six months old plant. The physiological effects of growth regulators Benylamino Purine (BAP), Thiadiazuron (TDZ), Kinetin (KIN), ? Naphthalene Acetic Acid full strength of Murashige and Skoog (MS) medium on in vitro propagation were investigated. The highest number of shoots in initiation (4.16 ± 0.33) and in sub-culture (15.22 ± 0.11) an maximum average shoot length (10.14 ± 0.03cm) were recorded on M S medium supplemented with BAP (1.0 mg/ml) at pH 5.8. Rooting was best achieved on M S medium augmented with NAA (2.0 mg/ml). The Plantlets in pots containing decomposed coir waste, vermiculite and garden soil (1:1:1 ratio) and grown in a shade house with 90.3% survival rate.

Key-Words / Index Term
In vitro, medicinal plant, hardening

How to cite this article
Siva Subramanian S., Krishnapriya A. And Narayanayyar V , “IN VITRO PROPAGATION OF ANISOCHILUS CARNOSUS (Lf.) Wallich – A MEDICINAL PLANT”, Journal for Advance Research in Applied Sciences, 4, Issue-7, pp.245-247, Dec 2017. DPI:16.10089.JARAS.V4.I7.2248