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MICROWAVE METHODS FOR THE EXTRACTION OF BIOACTIVE COMPONENTS AND ENZYMES FROM PINEAPPLE WASTE AND ITS APPLICATION IN MEAT TENDERIZATION
KAAVYA RATHNAKUMAR, KALPANA LAKSHMI, ANIL KUMAR ANAL

Published in: International Journal of Agriculture Sciences
Volume- 9, Issue-40, pp.4612-4620, Aug 2017
DPI :-> 16.10077.IJAS.2017.V9I40.46124620.2006



Abstract
The main purpose of this study is to extract the bioactive compounds from the pineapple waste using Microwave assisted extraction(MAE), as there is large amount of left over after processing which has potential uses. Bromelain a proteolytic enzyme found in the pineapple peel, stem, crown and core possess a lot of bioactive compounds like phenolics, antioxidants and enzymes which is useful in the food industry as well in pharmaceuticals. In this study MAE technique using water as solvent was employed to extract the bio actives and the proteolytic enzyme bromelain from the pineapple waste (peel and core) . Response surface methodology with Box Behnken design using the three independent variables time (min), power (watt) and solid to solvent ratio(g/ml) to determine the effect on the amount of Total protein, phenolic content and antioxidants (FRAP). Further using the optimized condition, the protein sample was purified using acetone to obtain Bromelain enzyme. This enzyme was freeze-dried, and applied to meat chunks as a tenderizer, and physiochemical parameters were determined and comparing it with the commercial bromelain from pineapple stem and results were analyzed.

Key-Words / Index Term
Microwave-Assisted Extraction, Pineapple waste, Response surface methodology Bromelain, Meat Tenderization

How to cite this article
KAAVYA RATHNAKUMAR, KALPANA LAKSHMI, ANIL KUMAR ANAL , “MICROWAVE METHODS FOR THE EXTRACTION OF BIOACTIVE COMPONENTS AND ENZYMES FROM PINEAPPLE WASTE AND ITS APPLICATION IN MEAT TENDERIZATION”, International Journal of Agriculture Sciences, 9, Issue-40, pp.4612-4620, Aug 2017. DPI:16.10077.IJAS.V9.I40.2006