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DETECTION OF AVIAN INFECTIOUS BRONCHITIS VIRUS BY RT-PCR AND RT-LAMP
AJAY DANDOTIYA, GURPREET KAUR, SREEKALA S. MOHANDAS, P.N. DWIVEDI

Published in: International Journal of Agriculture Sciences
Volume- 9, Issue-39, pp.4594-4597, Aug 2017
DPI :-> 16.10077.IJAS.2017.V9I39.45944597.1883



Abstract
Infectious bronchitis (IB) is caused by infectious bronchitis virus (IBV) belonging to family Coronaviridae. It is a highly contagious disease of poultry and is common throughout the world where poultry are produced commercially. PCR on reverse transcribed RNA is a potent technique for the detection of IBV. Tissue samples of Trachea, Kidney, Lung and Oviduct were collected from fifty layer birds and these were used for RNA extraction. The primers included S2F3 and S2B3 that amplify all IBV serotypes. The results of this study showed that 14% of the samples were positive to IBV by RT-PCR and RT-LAMP was optimized for further detection of IB virus.

Key-Words / Index Term
Poultry, IB virus, viral RNA, RT-PCR, RT-LAMP

How to cite this article
AJAY DANDOTIYA, GURPREET KAUR, SREEKALA S. MOHANDAS, P.N. DWIVEDI , “DETECTION OF AVIAN INFECTIOUS BRONCHITIS VIRUS BY RT-PCR AND RT-LAMP”, International Journal of Agriculture Sciences, 9, Issue-39, pp.4594-4597, Aug 2017. DPI:16.10077.IJAS.V9.I39.1883