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PRIMER DESIGNING AND VALIDATION OF STARCH PHOSPHORYLASE GENE FROM VIGNA SINENSIS VARIETY PUSA KOMAL
RANJEETA CHAUHAN, KRITYANAND KUMAR MAHATMAN

Published in: International Journal of Agriculture Sciences
Volume- 9, Issue-31, pp.4462-4464, Jul 2017
DPI :-> 16.10077.IJAS.2017.V9I31.44624464.1768



Abstract
Primer designing for Starch Phosphorylase Gene is done using Primer 3 software, with wet lab validation work has been carried out by PCR operation with agarose gel electrophoresis. Similar sequences for Starch Phosphorylase (Vigna sinensis) were retrieved from NCBI`s RefSeq database through Entrez, on multiple sequence alignment, conserved clusters were identified. The potential primers were 18-24 nucleotides selected in this region by all combination. The potential primer was analyzed using Primer Biosoft’s’ Net primer tool. The forward primer having 5` end and backward primer having 3` end and 50 to 60% GC content , and 54 to 580C Tm and without secondary structure were finalized. Specificity of the Primer was validated by carrying out the local alignment against the NCBI nr database through BLAST. All the alignment showed significant alignment to starch validating the specificity of the Primer.

Key-Words / Index Term
Starch phosphorylase, Primer designing, Vigna Sinensis

How to cite this article
RANJEETA CHAUHAN, KRITYANAND KUMAR MAHATMAN , “PRIMER DESIGNING AND VALIDATION OF STARCH PHOSPHORYLASE GENE FROM VIGNA SINENSIS VARIETY PUSA KOMAL ”, International Journal of Agriculture Sciences, 9, Issue-31, pp.4462-4464, Jul 2017. DPI:16.10077.IJAS.V9.I31.1768