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INVESTIGATION ON CALLUS FORMATION IN CAREYA ARBOREA ROXB
D. M. Wankhade, Dr. S. P. Rothe

Published in: International Journal of Current Engineering And Scientific Research ( IJCESR)
Volume- 5, Issue-1, pp.47-50, Jan 2018
DPI :-> 16.10046.IJCESR.2018.V5I1.4750.2314



Abstract
The purpose of this study was to standardize a medium protocol for induction and maintenance of callus from seeds of Careya arborea Roxb. Different types of media (Murashige and Skoog , 1962) were prepared according to various concentrations of growth regulators to standardize a medium protocol for callus induction from Careya arborea Roxb. The explants were surface sterilized, cultured and incubated at 24±2°C, under 16 hrs photoperiod, 8 hrs dark . The concentrations of MS medium for development of callus from the seed (explant) of Careya arborea Roxb at high frequency level hence, the medium protocol was standardized. Further, the same medium was used for maintenance of callus in vitro conditions.

Key-Words / Index Term
MS medium: Murashige & Skoog Medium, 2,4-D: 2, 4-diphenylphenoxyacetic acid, BAP: 6-benzylaminopurine, IAA: Indole-3- acetic acid, Con.: Concentration, KN: Kinetin, lit: litre, M: mole.

How to cite this article
D. M. Wankhade, Dr. S. P. Rothe , “INVESTIGATION ON CALLUS FORMATION IN CAREYA ARBOREA ROXB”, International Journal of Current Engineering And Scientific Research ( IJCESR), 5, Issue-1, pp.47-50, Jan 2018. DPI:16.10046.IJCESR.V5.I1.2314