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ANTICANCER ACTIVITY OF COLOCASIA GIGANTEA (BLUME) HOOK. f. IN CULTURED CELL LINES
Nambam Bonika Devi, Prof. Ganesh Chandra Jagetia

Published in: International Journal of Current Engineering And Scientific Research ( IJCESR)
Volume- 4, Issue-9, pp.1-11, Sep 2017
DPI :-> 16.10046.IJCESR.2017.V4I9.111.1911



Abstract
Evaluation of cytotoxic effects of the ethanol extract of Colocasia gigantea (CGE) in HeLa and V79 cells by MTT assay showed a concentrations dependent rise in the cytotoxicity. The maximum cytotoxicity was observed at 300 and 200 µg/ml ethanol extract of Colocasia gigantea for HeLa and V79 cells, respectively. Assessment of treatment duration revealed that cytotoxic effect of ethanol extract of Colocasia gigantea was marginal increased with treatment duration. Treatment of HeLa cells with different concentrations of ethanol extract of Colocasia gigantea reduced the clonogenicity of cells in a concentration dependent manner, which reached a nadir at 300 µg/ml. To understand the biochemical mechanism of action, the HeLa cells were treated with different concentrations of ethanol extract of Colocasia gigantea and contents of glutathione and activities of the glutathione-s-transferase and catalase and lipid peroxidation were determined. The ethanol extract of Colocasia gigantea reduced the glutathione concentration and activities of the glutathione-s-transferase and catalase in a concentration and time dependent manner and greatest reduction was observed at 6 h post treatment, whereas lipid peroxidation increased in a concentration and time dependent manner. The ethanol extract of Colocasia gigantea induced cytotoxicity and reduced the reproductive integrity of HeLa cells. The cytotoxicity of ethanol extract of Colocasia gigantean may be due to elevated lipid peroxidation and reduced concentration of glutathione and glutathione-s-transferase and catalase activities.

Key-Words / Index Term
Colocasia gigantea, MTT, clonogenic, GSH, GST and catalase.

How to cite this article
Nambam Bonika Devi, Prof. Ganesh Chandra Jagetia , “ANTICANCER ACTIVITY OF COLOCASIA GIGANTEA (BLUME) HOOK. f. IN CULTURED CELL LINES”, International Journal of Current Engineering And Scientific Research ( IJCESR), 4, Issue-9, pp.1-11, Sep 2017. DPI:16.10046.IJCESR.V4.I9.1911